Current Position
I work as the project manager for the Manchester Centre for Integrative Systems Biology (MCISB) at the University of Manchester, with Prof. Douglas Kell (MCISB director).
Research Interests
My main responsibility in the MCISB is the coordination of activities within our centre. The MCISB is pioneering the development of new technologies in Systems Biology, a new conceptual view on biological research that uses complex computational and mathematical analysis to advance traditional methods. We are developing a number a experimental and computational techniques for the study of the metabolism of bakers' yeast (Saccharomyces cerevisiae).
In addition, I am active in the creation of a network of SB research in Manchester and in the UK; here, our goal is to provide a hub for such activities, together with the other CISB centres in Newcastle, London (Imperial), Edinburgh, Oxford and Nottingham. In addition, we maintain close links with our colleagues in Amsterdam and in the industry.
To complement our research, the whole MCISB team including myself is involved in teaching in the DTC which is headed by Prof. Hans Westerhoff, here in the MIB.
Job Experience
| 2003-2005 |
Research assistant (Wissenschaftlicher Mitarbeiter) at the Max Planck Institute for Molecular Genetics in Berlin, Mass Spectrometry Group): Proteome analysis of mammalian cell lines; identification of proteins by peptide-mass-fingerpinting and MALDI-TOF-MS/MS. |
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| 1999-2003 |
Research assistant (Wissenschaftlicher Mitarbeiter) at the Freie Universität Berlin (Microbiology Department; Prof. Dr. Regine Hengge-Aronis); Establishment of analytical (quantitative) and preparative proteome analysis using 2D-gel electrophoresis and MALDI-TOF-MS; Teaching Microbiology to undergraduates and postgraduates; research into regulation and significance of proteolysis in Escherichia coli, in particular concerning the dynamic changes of protein levels during starvation and regrowth. |
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| 1996-1999 |
Postdoctoral position at the University of Wales (GB) in Aberystwyth (Dept. of Microbiology, Prof. Dr. Douglas B. Kell); Research project as part of the BBSRC Celsis Connect Programme using diode-array-HPLC and LC-ESI-MS for the analysis of novel natural organic substances; research into mechanisms of cell-to-cell signalling during recovery from starvation in Escherichia coli and other bacteria. |
Education
| 1996 |
Ph.D. in Microbiology at the University of Göteborg, Sweden; Title: "Survival of starved and nonculturable marine vibrios"; this included two years of research at the School of Immunology and Microbiology at the University of New South Wales in Sydney, Australia. |
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| 1990 |
Diploma in Microbiology at the University of Konstanz (Germany); Further subjects: Biochemistry, Genetics and Molecular Ecology. |
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| 1986 |
Conclusion of undergraduate (Vordiplom) studies in Biology at the University of Hamburg (Germany). |
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| 1982 |
A-level (Abitur) in Halstenbek (Schleswig-Holstein , Germany). |
Publications
Weichart, D., J. D. Oliver and S. Kjelleberg (1992): Low temperature induced non-culturability and killing of Vibrio vulnificus. FEMS MICROBIOL. LETT. Vol 100, pp. 205-210.
Kjelleberg, S., Albertson, N., Flaerdh, K., Holmquist, L., Jouper-Jaan, A., Marouga, R., Oestling, J., Svenblad, B., and D. Weichart (1993): How do non-differentiating bacteria adapt to starvation? ANTONIE VAN LEEUWENHOEK Vol. 63, pp. 333-341.
Kjelleberg, S., Flaerdh, K., Holmquist, L., Jouper-Jann, A., Olsson, R.M., Oestling, J., and D. Weichart (1993): Starvation and recovery of Vibrio . In: TRENDS IN MICROBIAL ECOLOGY (R. Guerrero und C. Pedros-Alio, Eds.) pp. 169-174.
Holmquist, L., Jouper-Jaan, A., Weichart, D., Nelson, D.R., and S. Kjelleberg (1993): Induction of stress proteins in three marine Vibrio species during carbon starvation. FEMS MICROBIOL. ECOL. Vol. 12, pp. 185-194.
Weichart, D., Lange, R., Henneberg, N., and R. Hengge-Aronis (1993): Identification and characterization of stationary phase-inducible genes in Escherichia coli . MOL MICROBIOL Vol. 10, No. 2, pp. 407-420.
Weichart, D., and S. Kjelleberg (1996): Stress resistance and recovery potential of culturable and viable but nonculturable cells of Vibrio vulnificus . MICROBIOLOGY ( UK ) Vol.142, No. 4, pp.845-853.
Paludan-Mueller, C., Weichart, D., McDougald, D., and S. Kjelleberg (1996): Analysis of starvation conditions that allow for prolonged culturability of Vibrio vulnificus at low temperature. MICROBIOLOGY ( UK ) Vol.142, No. 7, pp.1675-1684.
Otto, K., Weichart, D., and S. Kjelleberg (1997): Plasmid transfer between marine Vibrio strains during predation by the heterotrophic microflagellate Cafeteria roenbergensis . APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol.63, No.2, pp.749-752.
Weichart, D., McDougald, D., Jacobs, D., and S. Kjelleberg (1997): In situ analysis of nucleic acids in cold-induced non-culturable Vibrio vulnificus . APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol.63, No. 7, pp. 2754-2758 .
McDougald, D., Rice, S.A., Weichart, D., and S. Kjelleberg (1998): Non-culturability: adaptation or debilitation ? FEMS MICROBIOL ECOL. Vol. 25, pp. 1-9.
Marschall, C., Labrousse, V., Kreimer, M., Weichart, D., Kolb, A., and R. Hengge-Aronis (1998): Molecular analysis of the regulation of csiD , a carbon starvation-inducible gene in Escherichia coli that is exclusively dependent on s s and requires activation by cAMP-CRP. JOURNAL OF MOLECULAR BIOLOGY, Vol.276, No.2, pp.339-353.
Kell, D.B., Kaprelyants, A.S., Weichart, D.H., Harwood, C.R. and M.R. Barer (1998): Viability and activity in readily culturable bacteria: a review and discussion of the practical issues. Antonie van Leeuwenhoek , Vol. 73, pp. 169-187.
Davey, H.M., Kaprelyants, A.S., Weichart, D.H. and Kell, D.B. (1998): Approaches to the estimation of microbial viability using flow cytometry. In: CURRENT PROTOCOLS IN CYTOMETRY. Robinson P. (Ed.) Wiley, New York . Volume 11 (Microbial Cytometry) Section 11.3.
Barer, M.R., Kaprelyants, A.S., Weichart, D.H. Harwood, C.S., and Kell, D.B. (1998): Microbial Stress and culturability: conceptual and operational domains. MICROBIOLOGY ( UK ) Vol. 144, pp. 2009-2010.
Kaprelyants, A.S., Mukamolova, G.V., Kormer, S.S., Weichart, D.H., Young, M. and Kell, D.B . (1999): Intercellular signalling and the multiplication of prokaryotes: Bacterial cytokines. SYMP SOC GEN MICROBIOL Vol. 57, pp. 33-69.
Weichart, D.H., and Kell, D.B. (2001): Characterisation of an autostimulatory substance produced by Escherichia coli . MICROBIOLOGY ( UK ) Vol 147, pp. 1875-1885.
Weichart, D., Querfurth, N., Dreger, M., and Hengge-Aronis, R. (2003): Global role for ClpP-containing proteases in stationary phase adaptation in Escherichia coli . J. BACTERIOL. Vol. 185, pp. 115-125.
Stephani, K., Weichart, D., and Hengge-Aronis, R. (2003): Dynamic control of Dps protein levels by ClpXP and ClpAP proteases in Escherichia coli MOL MICROBIOL Vol. 49, 1605-1614.
Weichart, D., and Hengge, R. (2003): Physiologische Flexibilität durch regulierten Proteinabbau - Neue Dimensionen der Stress-Anpassung in Bakterien. BIOFORUM 11/2003, pp722-723.
Weichart D ., Gobom J ., Klopfleisch S ., Hasler R ., Gustavsson N ., Billmann S ., Lehrach H ., Seegert D ., Schreiber S ., Rosenstiel P . (2006): Analysis of NOD2-mediated proteome response to muramyl dipeptide in HEK293 cells. J. BIOL. CHEM. 281:2380-9.
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